Objective：To evaluate the biocompatibility of 3 kinds of artificial,biodegradable graft materials, including T20 [Poly （ butylene terephthalate）-co-poly （ cyclohexylene dimethanol terephthalate）-b-poly （ ethylene glycol）], PHBV （ Poly3hydroxybutyrate-Poly3-hydroxyvalerate） and PEGT/PBT [Poly （butylene terephthalate）/poly （ethylene glycol） terephthalate], and to study their effects on canine smooth muscle cells（SMCs） growth after surface modification. Methods： The contact angles were measured to compare the hydrophilicity of T20, PHBV, and PEGT/PBT scaffolds. Then SMCs were cultivated on these biodegradable scaffolds; their proliferation and growth were assayed by MTT assay, FITC and electron microscopic observation for evaluation of their in vitro biocompatibility. The growth of SMCs was also assayed on the 3 scaffolds after they were pretreated with gelatin or Poly-l-lysine. Results： The contact angles of 3 scaffolds were all less than 90°（PEGT/PBT〉T20〉PHBV）. MTT assay showed that SMCs adhered to and grew well on T20, PHBV, and PEGT/PBT scaffolds. The cell viability began to increase 24 h after cultivation （PEGT/PBT〉T20〉PHBV, P〈0.01）, followed by a decrease, but increased again 72 h after cultivation （T20〉PEGT/PBT〉PHBV, P〈0.01）. FITC observation showed that SMCs grew well and electron microscopic observation showed a confluent growth of SMCs with abundant extracellular matrix. The viability of SMCs was significantly higher on scaffolds pretreated with gelatin or Poly-l-lysine than on those without pretreatment（P〈0.01）. Conclusion： T20,PHBV,PEGT/PBT have good in vitro biocompatibility; the scaffolds made of these materials can obviously improve cell attachment after surface modification.