Objective：To determine the changes of mangiferin and neomangiferin contents in Rhizoma Anemarrhenae before and after processing. Methods： High performance liquid chromatography （HPLC） was used to determine the changes of mangiferin and neomangiferin contents in Rhizoma Anemarrhenae before and after processing. The mobile phase was ACN： 25 mmol/L KH2 PO4 （pH 3.0）, gradient elution. The Similarity Evaluation System for Chromatographic Fingerprint of TCM （2004 A edition） was used to evaluate the similarity of the 3 batches of Rhizoma Anemarrhenae before and after processing. Results： Mangiferin in the 3 batches was upregulated and neomangiferin was downregulated afer prosessing. The similarity of the 3 batches was all decreased（〈 1）. Conclusion： Processing changes the contents of mangiferin and neomangiferin in Rhizoma Anernarrhenae.