Objective：To study the factors influencing the amplification of sequence-related amplified polymorphism（SRAP） system in Carthamus tinctorius L. and to establish a stable SRAP reaction system, laying a foundation for molecular marker assistant breeding of Carthamus tinctorius L. Methods： Cetyltrimethylammonium bromide method was used to extract the genomic DNA of Carthamus tinctorius L.. Twenty-seven tests with 3 factors at 3 levels were designed, the conditions including Taq polymerase concentrations （0.02,0.04,0.06 U/μl）, dNTP concentrations （ 0. 15,0. 25,0.30 retool/L） and Primer concentrations （0.15,0.30,0.45 μmol/L） ;another 8 tests were designed based on the single factor of Mg^2＋ concentrations（0.5,1.0,1.5,2.0, 2.5,3.0,3.5 and 4.0 mmol/L）. Twenty ng DNA template was added into 25 μl SRAP reaction system; the system was optimized and agarose electrophoresis was used for determination. Results： A SRAP reaction system for Carthamus tinctorius L. was established. In a 25 μl reaction system, Taq polymerase was 0.02 U/μl, dNTP was 0.25 mmol/L,Primer was 0.30 μmol/ L, and Mg^2＋ was 3.0 mmol/L. Target bands increased after optimization, with good reproducibility, and the amplification results were satisfactory. Conclusion： The SRAP reaction system in this experiment is suitable for analysis of Carthamus tinctorius L.