Objective：To express and purify paired immunoglobin-like type 2 receptor beta （PILRβ） and prepare the polyclonal antibody against its protein. Methods： The PILRβ cDNA was amplified from the total RNAs extracted from human peripheral blood cells by RT-PCR and was cloned into pET-32a vector. The vector harboring PILRβ gene was then expressed in E. coli by IPTG induction and the product was purified by Ni^2＋-NTA affinity chromatography. Polyclonal antibody was prepared by immunizing rabbits with the purified protein. The titer and specificity of the antibody were examined by ELISA and Western blot, respectively. Results： Highly purified PILRβ protein （Mr 42 000） was obtained. The prepared polyclonal antibody was highly specific and had a titer of 1 ： 10 000. Conclusion： PILRβ gene is successfully cloned and the purified PILRβ protein is expressed. Polyclonal antibody against PILRβ（with high titer and specificity） is successfully obtained, which provides a basis for further study on PILRβ