Objective：To evaluate the perfusion defect of superficial lesions by highfrequency contrastenhanced ultrasound through comparing the results of lowfrequency and highfrequency contrastenhanced ultrasound, and to put forward the preliminary countermeasures. Methods: Twentythree patients (9 male and 14 female, aged 45.87±9.32 years, ranging 3473 years) with 6 sorts of superficial lesions, including 1 parotid pleomorphic adenoma, 3 thyroid adenomas, 5 thyroid adenocarcinomas, 5 cervical metastases, 7 breast carcinomas and 2 of testicular seminomas, were examined by both lowfrequency and highfrequency contrastenhanced ultrasounds. CEUS with SonoVue under CPSCadence mode was performed for each lesion using abdominal probe (lowfrequency probe) 5 min before small parts probe (highfrequency probe). ACQ software was used to determine the peak intensity (PI) and the results were analyzed by Student’s t test in pairs. Results: The PI values of all the 6 types of lesions were significantly lower in highfrequency contrastenhanced ultrasound images than those in the lowfrequency contrastenhanced ultrasound images (P<0.001). Conclusion: The microvascular enhancement of superficial lesions by highfrequency CEUS might be lower than the real enhancement intensity. Abdominal lowfrequency probe should be recommended for revealing the perfusion status of superficial lesions after highfrequency probe, which is used for observing the morphology and internal structures of superficial lesions.