Objective：To study the effect of resistin gene on TNFαinduced expression of ICAM1 in ECV304 cells. Methods: Resistin gene was cloned into vector pEGFPC1 to construct pEG/Resi plasmid; the latter was then used to transfect ECV304 cells via liposome.TNFα(20 μg/L) was added to the system 6 h after transfection. RTPCR was used to detect the expression of resistin and ICAM1 mRNA in the cells 18, 30, 42 and 54 h after transfection; meanwhile, the resistin protein levels were determined by Western blotting analysis. ICAM1 protein levels in the supernatants of ECV304 cells were determined by ELISA. Results: Restriction enzyme digestion and sequencing demonstrated that the recombinant plasmid was successfully constructed and was expressed in ECV304. ICAM1 levels in resistintransfected group were consistently higher than that in the nontransfected group in the presence of TNFα. ICAM1 mRNA and protein expression in the transfectedgroup was significantly higher than that in the nontransfected group during the peak periods (30 and 42 h) of resistin expression (P<0.05); ICAM1 mRNA and protein expression increased with the increase of resistin gene expression. Conclusion: Resistin gene can enhance TNFαinduced expression of ICAM1 in ECV304 cells.