Objective:To compare the eradication rates of H.pylori infection between a new sequential treatment regimen and conventional triple therapy in functional dyspepsia patients,and to assess the symptom improvement of functional dyspepsia.Methods: One hundred and thirty functional dyspeptic patients with H.pylori infection were randomly divided into 2 groups.Patients in sequential treatment group received 10-day sequential therapy:omeprazole (40 mg daily) plus amoxicillin (1 g twice daily) for the first 5 days,followed by omeprazole (20 mg),clarithromycin (500 mg) and tinidazole (500 mg) twice daily for the another 5 days; patients in triple therapy group received standard 7-day treatment:omeprazole (20 mg),clarithromycin (500 mg) and amoxicillin (1 g) twice daily for 7 days.H.pylori statuses were assessed by rapid urease test and 14C-urea breath test at base line level 4 weeks and one year after treatment，and symptom scores in various H.pylori statuses were evaluated.Results: The eradication rates of H.pylori in the sequential treatment group was 83.58%(56/67)by intention-to-treat(TTI)analysis and 91.80％(56/61) by per-protocol(PP)analysis,and the numbers in the triple therapy group were 65.08%(41/63)and 69.49%(41/59),respectively,which were significantly lower than those in the sequential treatment group (P<0.05). The symptom scores of patients with H.pylori eradicated after treatment were 4.88±1.76 before treatment and 2.18±0.83 one year after treatment (P<0.05); the scores of patients whose H.pylori was not eradicated after treatment was 4.99±1.66 before treatment and 3.89±1.56 one year later; there was no significant difference between the 2 numbers.Conclusion: Sequential treatment regimen has a better H.pylori eradication effect for functional dyspepsia patients positive of H.pylori than standard triple therapy; the symptom of dyspepsia can be greatly improved after H.pylori eradication．