Objective：To observe the influence of lentiviral vector-mediated RNA interference on expression of human APRIL (a proliferation-inducing ligand) gene in human pancreatic cancer cell line CFPAC-1, so as to pave a way for APRIL gene-targeted gene therapy of pancreatic cancer. Methods: Gene engineering technique was used to screen 3 RNA interference sequences targeting APRIL gene; the sequences were separately cloned into the pGCL-GFP vector to construct LV-APRIL shRNA1, LV-APRIL shRNA2 and LV-APRIL shRNA3, which were subsequently confirmed by PCR and DNA sequencing analysis. The titer of lentivirus was determined after 293T cells were cotransfected with LV-APRIL shRNA, pHelper 1.0 and pHelper 2.0. The 3 kinds of recombinant lentiviruses were injected into CFPAC-1 cells and the APRIL mRNA and protein expression were examined by real-time RT-PCR and Western blotting, respectively,and the result was compared with those of the non-transfected and blank vector transfected CFPAC-1 cells. Results: PCR analysis and DNA sequencing confirmed that the 3 APRILshRNA sequences were successfully inserted into the lentiviral vectors. The titers of concentrated virus were 5×107 TU/ml,6×107 TU/ml and 4×107 TU/ml, respectively. APRIL expression in CFPAC-1 cells was significantly inhibited at both mRNA and protein levels compared with the non-transfected and empty vector transfected CFPAC-1 cells (P<0.05). After transfection with LV-APRIL shRNA1 and LV-APRIL shRNA2, APRIL mRNA expression decreased by 73％ and 68％, APRIL protein expression decreased by 66％ and 59％ (P<0.05), respectively; there was no significantly difference between the non-transfected and empty vector transfected CFPAC-1 cells.Conclusion: Three lentiviral RNAi vectors of APRIL gene have been successfully constructed, and they can effectively inhibit the expression of APRIL gene in CFPAC-1 cells in vitro.