Objective：To study the effect of thermochemotherapy on lung cancer and its possible mechanism. Methods: H446 cells were subjected to different thermochemotherapy strategies: 43℃+Paclitaxel (120 μg/L,thermochemotherapy group), 43℃+Paclitaxel (120 μg/L)+SP600125 (20 μmol/L,JNK inhibitor) (thermochemotherapy+SP600125 group), thermotherapy (43℃) group, and Paclitaxel ( 120 μg/L) group; untreated cells served as control. MTT assay was used to measure cell proliferation and Western blotting was used to examine the expression of JNK, pJNK and HSP70 protein. Results: The proliferation rate of cells in the thermochemotherapy group was significantly lower than those in the other 3 groups (all P<0.05). The expression of pJNK was significantly increased in the thermochemotherapy group (P<0.05); SP600125 inhibited the expression of pJNK and the proliferation of cells in the thermochemotherapy+SP600125 group was elevated (P<0.05). The expression of HSP70 in the thermochemotherapy group was lower than that of the thermotherapy group (P<0.05).Conclusion: Thermotherapy can obviously promote the inhibitory effect of Paclitaxel chemotherapy against the growth of lung cancer cell line H446, probably through activating JNK pathway or inhibiting expression of HSP70 protein.