Objective:To evaluate the long-term safety of living kidney donors after nephrectomy. Methods:Twenty-six living donors of kidney were followed up for 2-8 years, with a mean of (4.27±2.11) years. The serum creatinine, blood pressure, proteinuria and psychological stress were observed in the donors during follow-up.Results: The serum creatinine had a significant increase after nephrectomy（P＜0.01）,but all had normal serum creatinine levels and remained stable thereafter. There was no significant change in blood pressure or proteinuria after nephrectomy;only one case developed microalbuminuria after nephrectomy.One of the donors regretted donation and felt more mental stress after nephrectomy. All of the donors were alive without complications.Conclusion: Pre-operation assessment and selection of donors, along with standard intra-operation management and long-term post-operation follow-up, can guarantee the safety of the donors.