Objective:To investigate the changes in common chemical signal molecules of neuroendocrineimmune (NEI) network and intervention of Tongxinluo (TXL,ultramicropulverization) of rats under hypoxia qiasthenia condition.Methods: Fortyfive male Wistar rats were randomly assigned to the following groups:control group,qiasthenia model group and TXL group (n=15).The rats in the former 2 groups were gavaged with CMCNa (10 ml/kg) once daily; those in the TXL group were gavaged with TXL (ultramicropulverization) suspended in CMCNa (10 ml/kg) once daily.After gavage,animals in the qiasthenia model group and TXL group were placed in a cabin with normal pressure hypoxia (7 h daily,6 d weekly for 5 weeks).At the end of the experiment,the biological characters of rats were observed and arterial blood gas was analyzed.The serum contents of serotonin (5HT),norepinephrine (NE),epinephrine (E),dopamine (DA) and IFNγ and TRH level in the hypothalamus were examined by enzyme linked immunosorbent assay (ELISA); the serum contents of corticosterone (Cort),T3,T4,IL1β,TNFα, and corticotropin release hormone(CRH) in the hypothalamus,and adrenocorticotropic hormone/thyroid stimulating hormone(ACTH/TSH) in the hypophysis cerebri were examined using radioimmunity method.Results: Compared with control group,the biological character scores of rats in the qiasthenia model group were significantly higher(P<0.01),arterial partial pressure of oxygen was significantly lower(P<0.05),the contents of 5HT,DA,NE and E were significantly decreased(P<0.01),the HPA and HPT systems were excited;adrenergic nerveadrenal medulla system was restrained and the release of IL1β,IFNγ and TNFα was significantly increased(P<0.01).The TXL could obviously decrease the biological character score and increase the arterial partial pressure of oxygen in model rats,raise 5HT and DA levels,restrain HPA and HPT system excitation,and decrease IL1β and IFNγ levels.Conclusion: The qiasthenia model can be successfully established using normal pressure chronic hypoxia.The NEI network regulation is chaotic and in a disequilibrium state,which might be an important basis for hypoxia qiasthenia syndrome.TXL can enhance the hypoxia tolerant capability of model rats and improve their qiasthenia syndrome,probably through adjusting NEI network disequilibrium.