Objective：To establish a nicotinetreatment and withdrawal rat model and to evaluate its characteristics and application through analyzing 3 parameters. Methods：Male SpragueDawley rats, aged 1011 weeks old, were randomly divided into normal saline group(subcutaneous injection of saline \[0.5 ml/kg\] for 6 weeks), nicotinetreated group (injection with nicotine \[0.5 ml/kg, 3 mg/（kg·d）\] for 6 weeks), and nicotinewithdrawn group (injection with nicotine \[0.5 ml/kg, 3 mg/（kg·d）\] for 3 weeks and followed by saline injection for additional 3 weeks). Body weight, food intake, and water intake of animals were recorded during the treatment in 3 groups. The model was evaluated through analyzing body weight, serum parameters and adipose tissue weights.Results：The body weight of rats, as well as the serum levels of triglyceride and insulin, were all decreased after nicotine treatment; the weights of subcutaneous fat, visceral fat and periaortic fat were also decreased. The above indicators increased after withdrawal of nicotine.Conclusion：The established model can be used to study multiple pharmacological effects of nicotine; it can also be used for smoking and smoking cessation related studies.