Objective： To screen,identify and classify an antimitosis marine bacterium strain 32-11-2-2 of Eastern China Sea. Methods： The marine bacteria were isolated with the infinite dilution and plate streak technique and screened for antimitotic active strains by Pyricularia Oryzae model. The active strain 32-11-2-2 was subjected to morphological, physiological and biochemical study, and was classified by the analysis of the 16S rDNA sequence. Results： A total of 421 strains of marine bacteria were isolated from marine water and sediment samples; 54 of them had antimitotic activity. The study of morphological, physiological and biochemical characteristics showed that the strain 32-11-2-2 was a slight halophilic strain; it had the morphological characteristics of a continent Bacillus and could produce amylase, fluidify glutin; and it also showed a positive reaction in the litmus milk test. The 16S rDNA analysis showed the strain 32-11-2-2 had a 98% homology with Bacillus subtilis. Conclusion： The strain 32-11-2-2 has been identified to be a Bacillus subtilis adapted to the oceanic environment.