Objective：To investigate the influence of C-type natriuretic peptide（CNP） on the expression of natriuretic peptide B receptor（NPR B） and its cGMP signal induced by advanced glycation end products（AGEs） in human mesangial cells（HMC）. Methods： HMCs were cultured in vitro. The expression of NPRB, intracellular concentration of cGMP and level of NF-κB p65 in HMC were measured by immunofluorescent staining, enzyme linked immunosorbem assay（ELISA） and flow cytometry, respectively. Results： CNP significantly upregulated the immunofluorescent intensity of NPR-B and the concentration of cGMP inhibited by AGEs（P〈0.001） ;CNP also inhibited the fluorescent intensity of NF-κB p65 stimulated by AGEs. Conclusion： CNP can regulate the expression of NPR-B/ cGMP and activity of NF-κB induced by AGEs through a NPR- B/cGMP dependent manner.[著者文摘]