Objective:To establish a method for isolating and culturing non-small cell lung cancer-derived vascular endothelial cells (NSCLCDVECs) and to observe their characteristics in vitro.Methods: Enzyme digestion method was used to isolate microvascular fragments from human non-small cell lung cancer tissues,and explant culture method was used for primary culture of the microvascular endothelial cells,then the cells were purified by local digestion and differential adhesion method.The obtained NSCLCDECs were identified by optical microscope,immunocytochemistry and immunofluorescence methods.Results: The NSCLCDECs were positive for FⅧ-RAg and CD34.Microscopy showed that the cells grew well and could be passaged.Conclusion: We have established a method for isolating and culturing NSCLCKVECs,and the resultant cells possess a great value for research on anti-vascularizaton and anti-tumor agents.