Objective：To design and prepare a fiberoptic bronchoscopy training box and to study its primary application.Methods: According to the length of optical fiber,the height,the length and the width of the box were set at 400 mm,200 mm and 200 mm.The box contained 6 layers from the top to the bottom:the top-layer,layer-A,layer-B,layer-C,layer-D and the bottom-layer,with the area of each layer being 200 mm×200 mm and the distance between each 2 layers was about 80 mm.According to the diameter of optical fiber,the diameter of the holes was set at 6 mm.Except for the bottom-layer,the other 5 layers had different numbers of holes:the top-layer and layer-A only had a central hole(central hole in every layer was defined as hole-0),layer-B had 5 holes(hole-0 to 4),layer-C had 9 holes(hole-0 to 8),layer-D had 17 holes(hole-0 to 16).The training started from the top-layer.The orders were given according to the layers and the numbers of the holes and the orders were executed,then the box was open to examine the outcome of the execution.Results: We successfully designed a fiberoptic bronchoscopy training box.If the training instruction order was A0-B2-C4-D7,the trainees should manipulate the optical fiber via the central hole in top-layer and layer-A,hole-2 in layer-B,hole-4 in layer-C,and hole-7 in layer-D.The results of manipulation can be examined after opening the door.Conclusion: The fiberoptic bronchoscopy training box is a easy-to-operate and practical tool for training of fiberoptic bronchoscopy manipulation.