Objective：To study the influence of Wnt5a gene silence on the proliferation,migration and invasion of lung squamous carcinoma cells.Methods: A recombinant plasmid pH1-siRNAWnt5a- was constructed and used to deliver small interference RNA (siRNA) targeting Wnt5a in SK-MES-1 cells; the transfected cells were screened to establish a stable transgenic cell line.MTT,cell cycle and Transwell assays were employed to evaluate the effect of Wnt5a gene silence on the proliferation,migration and invasion of lung squamous carcinoma cells.Results: Western blotting assay revealed that Wnt5a was lowly expressed in SK-MES-1Wnt5a-(13.6%).The proliferation index (PI) of transgenic cell line was slightly lower than that of the control cell line (\[28.3±3.8\]% vs \[30.5±5.2\]%).The migration and invasion capabilities of SK-MES-1Wnt5a- cells were (47.3±9.2)% and (39.7±11.7)% of the control cells,respectively.Conclusion: Low Wnt5a expression can significantly inhibit the migration and invasion capabilities of SK-MES-1 cells,indicating that Wnt5a might be a potential target for gene therapy of lung squamous carcinoma.