Objective:To develop valved aortic stent and to assess the feasibility of its trans-catheter implantation to the ascending aorta in vivo. Methods: We designed self-expandable nitinol stents (15-19 mm in diameter) according to the internal diameter of the ascending aorta of Chinese miniswines. Bovine pericardium was cross-linked with 0. 6% glutaraldehyde solution for 36 hours, and then was shaped and sutured into the stents. Fluid passing test, pre-releasing test and static test of pressure in the tube were performed in all devices. The devices were tried to be inserted percutaneously through common iliac artery to deploy in the ascending aorta in 7 Chinese miniswines. Results: In vitro test showed that the closure of the percutaneous valved aortic stent leaflets was satisfactory, and the fluid flow was not restricted in the opposite direction. The devices could be released through the catheter, expanded completely, and be fixed rapidly in the tube. The devices were deployed using 12F-16F catheter and were inserted through common iliac artery in 6 of the 7 Chinese miniswines. All devices were implanted in the desired position, with 6 expanded completely and fixed rapidly in the ascending aorta. One did not expand completely and was displaced. Animals were executed and dissected after 24 hours. All the devices were fixed in the ascending aorta and there were no thrombus. Conclusion: The function of the percutaneous valved aortic stent is satisfactory. The size of compressed device is suitable for catheterization. It can be successfully implanted into the ascending aorta by using a retrograde method through common iliac artery.