Objective:To observe the differential expression of epithelial membrane antigen (EMA) and renal cell carcinoma marker (RCC-Ma) in early renal carcinoma and corresponding adjacent (0.5, 1.0, and 2.0 cm) tissues, so as to estimate the safe margin of laparoscopic partial nephrectomy for renal tumors.Methods:Tissue microarrys, including 44 tissues of clear cell renal cell carcinoma［A］ and normal renal cortex tissues of different distances to the tumor：0.5 cm［B］，1.0 cm［C］ and 2.0 cm［D］, were constructed.The expression of EMA and RCC-Ma was examined by immunohistochemical staining in the four groups. Results:Intensive membranous and(or) cytoplasmic staining of EMA and RCC-Ma was observed in the cancer and adjacent tissues. The positive rate in group A was significantly higher than those of the other three groups（A \[15.91%/18.18%\]，B\[84.09%/79.55%\]，C\[86.36%/77.27%\] and D\[79.55%/75.00%\], P<0.01）.The positive rates of EMA and RCC-Ma expression were not significantly different between the group B, C, and D（P>0.05）.Conclusion:Our data denote that, when laparoscopic partial nephrectomy is done for early renal carcinoma, at least 5 mm of normal parenchyma tissue beyond the pesudocapsule should be excised with the tumor.