【ABSTRACT】： Objective : To establish a SYBR Green quantitative real-time PCR method to monitor the dynamics for NF-κB mRNA and IκB mRNA of opened abdominal injury with seawater immersion in rats. Methods: Wistar rats were randomly divided into three groups: the control group(n=7), the opened abdominal injury groups(n=28, all rats were randomly divided into four groups separately , which groups were 12h, ,24h, 48h ,and 72h after wounded) and opened abdominal injury with seawater immersion groups(n=28,a all rats were randomly divided into four groups separately , which groups were 12h, ,24h, 48h ,and 72h after wounded).The dynamics for the express of NF-κB mRNA and IκB mRNA of small intestine tissues was detected in every groups by real-time PCR method.. Results: NF-κB mRNA level was rise obviously after 72h in the opened abdominal injury with seawater immersion groups than other groups in same time point（P＜0.01）.The expression of IκB mRNA level was rise obviously after 48h in the opened abdominal injury with seawater immersion groups compared with the opened abdominal injury groups after 48h（P＜0.01）. Conclusions: The NF-κB signal pathways was activated and magnified in latter periods of the opened abdominal injury with seawater immersion（＞72h）, Which led to the development of continued inflammatory course ultimately.