Objective To investigate the effect of siRNA targeting TGF-β inducible early gene (TIEG) on expression of Smad2 in advanced glycation end products (AGEs)-mediated renal tubular epithelial cells. Methods The pshRNA-copGFP-lentivector containing target gene was used as a vector to construct siRNA-TIEG, which was then transfected into normal rat proximal tubular epithelial cells(NRK52E),which were then cultured in RPMI 1640 medium supplemented with AGE-BSA for 24 h and 48 h. The expression of Smad2 mRNA and protein was examined by PCR, Western blotting analysis, respectively. Blank vector group served as control.Results SiRNA-TIEG significantly reduced the expression of TIEG mRNA, Smad2 mRNA and Smad2 protein in normal rat proximal tubular epithelial cells in presence of AGE-BSA compared with those in the control group (P<0.05). Conclusion Silence of TIEG can effectively inhibit the expression of Smad2 mediated by AGEs in NRK52E cells.