ObjectiveTo study the effect of metastasis suppressor gene Kai1/CD82 on the cell proliferation of Hep-2 cell line.MethodsRecombinant adenovirus rAd-Kai1 was amplified,purified,and was used to transfect laryngo-carcinoma Hep-2 cell line.Cells transfected with blank vector and untransfected cells served as controls.MTT assay was used to assess the influence of Kai1 gene on proliferation of Hep-2 cells; RT-PCR was used to examine the expression of Kai1 gene in cells of different groups.ResultsThe titer of rAd-Kai1 reached 6×1010PFU/ml after expansion and purification.When the concentration of the adenovirus was 30 MOI,the transfection rate of Hep-2 cells could be higher than 90%.MTT assay showed that the cell proliferation abilities were similar in the two control groups,and the proliferation ability of cells in the Kai1 transfection group was significantly lower than that in the non-transfected group (P<0.05),with the inhibitory rate in the Kai1 group being 29%.ConclusionThe Kai1/CD82 gene can inhibit the proliferation of Hep-2 cells.