Objective To try to perform single-port laparoscopic nephrectomy in pigs, and to summarize and improve the basic skills, so as to decrease risks in clinical practice. Methods From Apr. to May 2009, we performed 10 times (5 pigs) of nephrectomy on porcine model using Triport system. The experience was summarized with respect to the following issues: the choice of endoscopy and instruments, insertion of Triport single port system, and optimization of the basic operation procedure. Results All the procedures were successful, without bleeding, organ injury or other complications. Operative time decreased from 75 min at the first time to 23 min for the last one. Clashing of instruments was the biggest frustration in the process; “X” shape crossing can partially resolve the problem, based on which four kinds of basic operating modes were designed. Conclusion Single-port laparoscopy nephrectomy in pigs can help the new learners to quickly improve their skills and decreases clinical risks.