Objective To observe the inhibitory effects of matrine, oxymatrine and sophordine on expression of CD91 and CD13 and secretion of TNFα in macrophage RAW264.7. Methods Macrophages were cultured in 6hole plate and coverslip was prepared. Then macropages were cultured one day with different concentrations(0,12.5,25,50,100,200 mg/L) of matrine, oxymatrine and sophordine; the expression of CD91 and CD13 by immunohistochemical dyeing was analyzed by ImagePro plus system (Media Cybemetic, Inc., 6.0 edition). Meanwhile, the levels of TNFα in the culture medium of macrophages were examined by ELISA. Results Matrine, oxymatrine and sophordine could all inhibit CD91 and CD13 expression in macrophage RAW264.7, and the inhibition varied with the concentrations of matrine, oxymatrine and sophordine; the inhibitory effect of matrine was the most prominent one. Moreover, matrine, oxymatrine and sophordine also inhibited the secretion of TNFα. Conclusion Matrine, oxymatrine and sophordine can greatly inhibit expression of CD91 and CD13 and secretion of TNFα in macrophage RAW264.7.