ObjectiveTo investigate relationship of GSTP1,RASSF1A polymorphisms and environmental agent with susceptibility to prostate cancer (Pca).MethodsThe GSTP1 and RASSF1A genotypes were determined by TaqMan/MGB Probe Technology in 103 patients with Pca and 103 normal controls. Multivariate logistic regression model was used to assess the association of smoking,alcohol drinking,tea drinking,weekly pork and beef consumption,and the genetic polymorphisms with the susceptibility to Pca,while taking into consideration of the environmental agent.ResultsThe frequencies of the GSTP1 AA,AG and GG genotypes were 66.02%,22.33%,and 11.65% in patients with Pca and 67.96%,29.13% and 2.91% in controls,respectively,with significant difference found between the two groups (χ2=6.35,P=0.04). The frequencies of RASSF1A CC,CA and AA genotypes were 88.34%,5.83%, and 5.83% in patients with Pca,and 85.44%,12.62%, and 1.94% in the controls,respectively,with no significant difference found between the two groups (χ2=4.63,P=0.10).Multivariate analysis showed a decreased risk in those who had a tea drinking history (OR=0.40,95%CI,0.19-0.82) and an increased risk in those who had a smoking history (OR=3.02,95%CI,1.44-6.32).ConclusionOur results indicate that GSTP1,RASSF1A polymorphisms are not associated with Pca susceptibility in Chinese Han nationality. Smoking is the risk factor of Pca,and tea drinking is a protective factor against Pca.