ObjectiveTo predict the B cell epitopes of tumor-associated protein EIF4G1 subtypes.MethodsThe sequences of all the protein subtypes of EIF4G1 were retrieved from NCBI protein database.Based on single parameter evaluation,including hydrophilicity,flexility,antigenicity,the B cell epitopes of the EIF4G1 protein subtypes were predicted using NPS@structure software and ABCpred software.ResultsEIF4G1 protein had five subtypes.The variation of the five different EIF4G1 subtypes was limited within a 300aa region.We identified eight epitopes locating in or near 14-19,21-27,52-61,106-112,113-139,183-189,201-216, and 217-224, which can be used to identify specific B cell epitopes of different protein subtypes.ConclusionB cell epitopes of EIF4G1 protein subtypes do exist,and they may be used for the protein subtypes evaluation and early diagnosis of tumor patients using artificially-produced matched peptides.