\[Abstract\]ObjectiveTo investigate the effect of insulin-like growth factor-1 (IGF-1) on expression of cystationine-β-synthase(CBS)and hydrogen sulfide(H2S) in rat adrenal pheochromocytoma cell line (PC12) and the possible signaling pathway. MethodsAfter PC12 cells were treated with 20,40, or 80 ng/ml IGF-1 for 24 h,fluorecence quantitative PCR was used to examined the expression of CBS gene, Western blotting analysis was used to detect the expression of CBS,ERK/MAPK protein expression, and sensitive sulphur electrode was used to analyze the levels of H2S gas. For inhibition studies,25 or 50 mol/L PD98059,a specific ERK/MAPK inhibitor,was used 30 min prior to 80 ng/ml IGF-1 treatment; and the above parameters were examined again using the same method. ResultsIGF-1 increased the expression of CBS,pERK1/2, and the levels of H2S；and PD98059 could inhibit the above effect of IGF-1.ConclusionIGF-1 and its ERK/MAPK signaling pathway regulate the expression of CBS and levels of H2S.