ObjectiveTo use the pooled sampling method to reduce the error caused by the detecting precision, so as to improve the accuracy of population rate estimates. MethodsA formula was deduced on how to obtain the ideal rate under given sensitivity and specificity, and the mean relative errors of rate estimation with different pool sizes were simulated and calculated using software SAS 9.1. ResultsWhen the actual rate was lower than the ideal rate, the errors caused by the detecting precision could be greatly reduced through adjusting the rate of mixed samples. ConclusionWhen the population rate is low, the accuracy of rate estimation can be improved and the numbers of tests can be reduced by using pooled sampling method under given specificity and sensitivity.