\[Abstract\]ObjectiveTo set up a baculovirus system for preparing recombinant adeno-associated virus serotype 8 (rAAV8) and to preliminarily examine the infection viability of the prepared virus in vitro. MethodsThe rAAV8-EGFP was prepared using baculovirus system and was subsequently extracted and purified by high performance liquid chromatography. The viral titer was determined by real-time quantitative PCR and its infection viability for HEK-293 cells was examined by observing the EGFP reporter. ResultsReal-time quantitative PCR showed that high titer of rAAV8-EGFP was prepared (1.5×1013 vg/bottle\[100 ml medium\]) and strong expression of EGFP was observed in HEK-293 cells infected with rAAV8-EGFP. ConclusionThe baculovirus system is capable of producing rAAV8 with high titer and infection viability, paving a way for future research.