\[Abstract\]〓Objective〓 To improve the staining efficiency and quality of biological specimens ultrathin section for transmission electron microscopy. Methods〓 The “plugin staining method” and continuous staining were developed by modifying the traditional staining method. Results〓 Using our method, we completed the electron staining of a large number of ultrathin sections of biological specimens of within a short period of time. 〖WTHZ〗Conclusion〓 Compared with traditional method, the new method can save time and drug, and reduce the possibility of staining contamination. The ultrastructure observed by electron microscope is more clear and distinct.