ObjectiveTo observe the expression of microRNA-200c (miR-200c) in ovarian cancer cell line and tissues, and to assess its possible clinical value. MethodsReal-time PCR was used to analyze the expression of miR-200c and miR-141 in HO-8910 (human ovarian cancer cell line), HO-8910PM (highly metastatic ovarian cancer cell line), and HO-8910 cluster cells. We also determined the expression of miR-200c and miR-141 in 83 ovarian cancer tissues, 13 borderline ovarian tumors, and 8 normal ovary tissues; the expression was compared between tumors of different pathological characters. ResultsExpression of miR-200c was down-regulated in HO-8910 cluster cells compared with those in HO-8910 and HO-8910PM cells(P＜0.05).Expressions of miR-141 and miR-200c were gradually up-regulated from the normal ovary tissue to borderline ovarian tumors, then to ovarian cancer tissues (P<0.05). Expression of miR-200c was down-regulated in the metastatic ovarian cancer and ovarian clear cell tumors and undifferentiated ovarian cancer cells(P<0.05). Patients with high miR-200c expression had a better prognosis than those with a low expression of miR-200c (P<0.05). Conclusion The decreased expression of miR-200c is correlated with the progression of ovarian cancer patients, and it is a risk factor of poor prognosis.