ObjectiveTo analyze the topological properties of genomic-wide genetic interaction network in yeast. MethodsThe topological properties of genomic-wide genetic interaction network in yeast were calculated by the graph theory. ResultsA power law fit the degree distribution well in the genomic-wide genetic interaction network in yeast, with the exponent approaching 3. The Node’s degree followed a wide-tail distribution, with the average degree being 87. Two thirds of the genes had genetic interaction via only one gene, and about one third of the genes had genetic interaction via only 2 genes. The average clustering coefficient was 0.047. ConclusionThe gene function in yeast is usually multiple and the functional interaction between genes is highly condensed.