Objective To study the effect of Danshen on the pharmacokinetics of Rosuvastatin in rats. Methods Parallel control method was applied in this study. Twelve male rats weighing (230±15) g were evenly randomized into two groups: rosuvastatin (80 mg/kg) group and Rosuvastatin plus Danshen (400 mg/kg) group. Blood samples (0.3 ml each) were collected from the orbital vein at 0.5, 1, 1.5, 3, 5, 8, 10, 12 and 24 h after drug administration. LC-MS was used to determine the plasma concentrations of Rosuvastatin. The pharmacokinetic parameters of the two groups were compared and statistical analysis was performed using SPSS12.0 software. Results When combined with Danshen, the Cmax, AUC0-t, and AUC0-∞ of Rosuvastatin were increased by 60.81%, 88.00% and 82.92%,and T1/2 and CLz/F were reduced by 33.21% and 21.51%, respectively. Conclusion Danshen can affect the pharmacokinetic properties of Rosuvastatin in rats.