Objective To evaluate the feasibility of using the ultrasound-guided vacuum-assisted breast biopsy system (Mammotome) for diagnosis and minimally invasive resection of calcification in the breast. Methods Fourteen patients with breast calcification lesions who were diagnosed by mammography were resected by ultrasound-guided Mammotome system with 8G probe. The samples were subjected to mammography, followed by pathological examination. Results All the lesions were removed accurately and radically with satisfactory cosmetic outcomes; there were no clinically significant hematoma after operation. Each lesion was resected via (9±2.22) peelings and took a mean of (12.93±3.45) min．Pathological examination confirmed 2 cases of ductal carcinoma in situ, 5 cases of fibroadenoma, and 7 cases adenosis. Conclusion Ultrasound-guided Mammotome system can be used for diagnosis and treatment of breast calcification lesions which are ultrasound positive. The technique is accurate, time-saving, and mini-invasive, but it requires more experience and patience from the operators.