Objective To create a simple model for teaching laparoscopic partial nephrectomy(LPN) in a simulated continuous blood supply setting and to introduce the main technical points of the procedure. Methods A fresh porcine kidney was placed on an unfolded metallic box,and the renal artery was infused with red-dyed water to simulate the blood supply of the kidney. The specially designed metallic box could not only fix and position the kidney, but also store the outflow of the kidney while the artery was kept artificially infused. The trainees were required to excise about 2 cm spherical renal parenchyma tissue and to suture the pelvis and renal parenchyma. The trainees had a similar laparoscopic competency and they all completed the dry laboratory training programs. During a 20-day training period, the trainees were assessed by the time periods they needed to complete LPN. The procedure and the quality of the LPN were monitored by the training mentors.Results The mean operating time decreased from (46.1±1.64) min (range, 43-49 min) before training to (27.9±1.79) min (range, 25-31 min) after training (P<0.001). The operating time required for suture-repairing the pelvis and renal parenchyma began to decrease after performing the fourth procedure, with the time decreased from (30.4±1.82) min to (19.4±1.16) min (P<0.001). The mean quality score increased from 2.02±0.19 at the beginning to 4.41±0.14 at the end of the training (P<0.001). The increase of the quality score was faster during the first five procedures. All the trainees became confident in performing a high quality LPN after completing ten procedures. ConclusionThe present model with isolated porcine kidney is simple and economic. It can offer the trainees the opportunities to learn all the skills necessary for LPN.