ObjectiveTo investigate the effect of down-regulated S100A7 expression on proliferation and migration of gastric carcinoma SGC-7901 cells, and to explore the possible molecular mechanisms. MethodsThe experiment was divided into three groups, including untransfected group, control siRNA group and S100A7 siRNA group. S100A7 siRNA and control siRNA were transfected into gastric carcinoma SGC-7901 cells in S100A7 siRNA group and control siRNA group, respectively. Expressions of S100A7 mRNA and protein in gastric carcinoma SGC-7901 cells were examined by Real-time PCR and Western blotting analysis, respectively. CCK-8 kit and Boyden chamber were used to observe the proliferation and migration of SGC-7901 cells in the three groups. The expressions of cyclin D1, Cdk2 and MMP-2 proteins were also examined by Western blotting analysis in the three groups. ResultsS100A7 siRNA effectively down-regulated the expressions of S100A7 mRNA and protein in gastric carcinoma SGC-7901 cells. Down-regulated S100A7 expression greatly inhibited the proliferation and migration of gastric carcinoma SGC-7901 cells, and also down-regulated expression of cyclin D1, Cdk2 and MMP-2 proteins. ConclusionDown-regulated S100A7 expression-mediated inhibition of gastric carcinoma cell proliferation and migration may be closely associated with the decreased expressions of cyclin D1, Cdk2 and MMP-2 proteins.