Objective To evaluate the value of flexible ureteroscopy (F-URS) in managing asymptomatic calyceal calculi for flight attendants. Methods We retrospectively analyzed the clinical data of 8 flight attendants who were surgically treated for asymptomatic calyceal calculi at our hospital from January 2008 to May 2012. The demographic information, disease characteristics, and perioperative information were collected. Results Eight patients had a total of 37 intrarenal calculi, with bilateral renal stones found in 2 patients. The mean number of stones per kidney in our patients was 3.7±0.7 (range 3-5). The mean size of individual stone was (4.5±1.5) mm (range 2-8 mm). The mean stone size per kidney was (16.8±4.7) mm (range 6-20 mm). The mean operation time was (45.0±7.6) min (range 30-56 min) and the average postoperative hospital stay was (1.8±0.5) d (range 1-2 d). Mean time lost from work due to F-URS was (13.0±1.1) d (range 12-15 d). No severe complications occurred during the operation. Stone free rate was 100% at 2 weeks after treatment as showed by a follow-up CT scan. Conclusion F-URS with holmium laser is a safe, effective and minimally invasive procedure, and may serve as a first-line therapy for asymptomatic calyceal calculi for flight attendants.