Objective To investigate the feasibility of per-left auricle suture of mitral valve using modified vascular closure device via in vitro experiment, so as to search for new technique for treatment of mitral regurgitation. Methods The market vascular closure device was modified by cutting the tip of extension tube, while reserving the marking hole for passing the puncture wire and broadening the needle pitch. Twenty goat hearts were obtained and the pericardium was opened to expose the left auricle. After puncture of the left auricle with the Cordis needle, a “J” type guide wire was inserted through the needle sheath. It was confirmed that the “J” type guide wire must be in the left ventricle through cutting the left ventricle along the interventricular groove. Then the modified vascular closure device was introduced into the left ventricle along the “J” wire. The anterior leaflet and posterior leaflet of mitral valve were sutured together by the vascular closure device in ventricular side of the mitral valve. Results Our modified vascular closure device could successfully suture the anterior leaflet and posterior leaflet of mitral valve, without damaging the valve structure. Conclusion The modified vascular closure device is well designed and can be used for suturing anterior leaflet and posterior leaflet of mitral valve.