Objective To use ultrasound-guided percutaneous microwave for simultaneous ablation of a nodule in submandibular gland and another in the ipsilateral thyroid gland. Methods A 57-year old man presented non-painful nodules in his right submandibular gland and right thyroid lobe simultaneously, with the sizes being 10 mm×10 mm×9 mm and 13 mm×11 mm×10 mm separately. Percutaneous microwave ablation and biopsy were conducted for both nodules simultaneously under ultrasound guidance. The short-term effects were documented both clinically and ultrasonographically. Results The ablation therapy was completed uneventfully and took a total of 20 min. The ablation time was 185 seconds for the submandibular nodule and 72 seconds for the thyroid nodule. No intra-operative complications such as bleeding, edema or nerve injury were observed. No symptoms of injuries to the facial nerve, glossal nerve or recurrent laryngeal nerve were observed during a 2-month follow-up. Ultrasound examination revealed that the ablated area was shrank and kept unenhanced on CEUS. Conclusion Microwave ablation therapy is suitable for the conjoint treatment of concomitant nodular lesions in multiple neck organs. The method is economical, efficient, minimally invasive, and is worth of more clinical practices.