Objective To evaluate the clinical efficacy of silver-containing Hydrofiber^R○ dressing in sealing the donor sites of the split-skin by comparing with traditional petrolatum gauge. Methods A prospective case-control study was performed from March 2012 to March 2013 in Department of Burn, Changhai Hospital. A total of 30 patients fulfilling the inclusion criteria were allocated into two groups according to the methods of sealing the donor sites. The donor sites of the study group(10 patients) were treated with silver-containing Hydrofiber^R○ dressing and those in the control group (20 patients) were treated with petrolatum gauge. For each study case, two cases with similar diagnosis were allocated as controls. The following data were recorded: demographic data, condition of donor sites, infection rate of the donor sites, time for re-epithelization of the donor sites, time of first postoperative dressing change, pain score and number of gauges soaked with blood during first postoperative dressing change.Results The study group needed a significantly shorter time for re-epithelization of the donor sites compared with the control group (\[9.60±0.84\] d vs \[10.90±1.02\] d,P<0.05). The study group also exhibited significantly lower pain score during first postoperative dressing change as compared with the control group (1.50±0.71 vs 3.75±0.79,P<0.05). The time interval between skin grafting and first postoperative dressing change in study group was significantly longer than that in the control group (\[7.30±0.48\] d vs \[5.45±1.64\] d,P<0.05). The number of gauges soaked with blood at the first postoperative dressing change in study group was significantly less than that in the control group (1.00±0.67 vs 3.10±0.85,P<0.05). Wound infection was not found in both study group and control group. Conclusion Silver-containing Hydrofiber^R○ dressing applied for the donor site of the split-skin can promote the healing of the donor sites, reduce local pain, and have satisfactory antiseptic and hemostatic effects, with acceptable therapeutic outcomes.