Objective：To estimate the effect of docetaxel combined with cyclopamine on the treatment of glioma. Methods The anti-proliferation efficiency of DOC combined with cyclopamine was evaluated by MTT assay. Tumor spheroids were used to evaluate anti-tumor ability of DOC DOC combined with cyclopamine. U87 cells were xenografted in athymic nude mice to establish the animal model, which were used to evaluate the effect of anti-glioma. Results The anti-proliferative activity against U87s and tumor spheroids confirmed strong inhibitory effect of docetaxel combined with cyclopamine. The combination of DOC and cyclopamine can prolong the median survival time of U87 bearing nude mice than other groups significantly. Conclusion the DOC and cyclopamine can inhibit the growth of gioma, and the combination of DOC and cyclopamine may be a potentially effective treatment for glioma.