Spinal fusion is the common procedure to restore the stability of spine. However, fusion failure and pseudoarthrosis are frequent. Previous studies have found that low-intensity pulsed ultrasound (LIPUS) can accelerate spinal fusion and reduce fusion failure, with endochondral ossification being the main pathological process. Further investigations have revealed that LIPUS can directly promote the migration, proliferation, osteogenesis, and chondrogenesis of mesenchymal cells. Moreover, LIPUS is indicated to accelerate angiogenesis and innervation coupling, finally promoting spinal fusion.