Objective To evaluate the efficacy and safety of the robotic-assistant laparoscopic pyeloplasty(RALP) combined with flexible ureteroscopy(f-URS) for treatment of ureteropelvic junction obstruction(UPJO) with renal stones. Methods We retrospectively reviewed the records of 5 patients who underwent RALP and f-URS for UPJO combined with renal stones from May 2012 to August 2013.Three cases had solitary stones located at the lower calyx, and the other two had multiple stones. Results The operation was successful in all the 5 patients with no severe complications. The average total operation time was (132.4±24.8) min and the average f-URS time was (25.4±11.9) min. f-URS had full views of the collecting system with no blind area in all cases. Post-operative KUB and CT scan showed no residual stone fragments. The symptoms were greatly relieved in all patients after a 6-month follow-up postoperatively, with one patient still having 2 cm hydronephrosis; and the UPJO outcomes were satisfactory in the others. Conclusion Hybrid RALP combined with f-URS is safe and feasible for treatment of UPJO complicated with renal stones.