Objective To evaluate the liver cooling effect of infusing cold fluid through the bile duct in liver injury models. Methods A total of 14 BA-MA mini-pigs were randomized into two groups: control group (n=7) and hypothermia group (n=7). All the animals underwent tracheal intubation under general anaesthesia and liver injury was created after opening the abdomen. The liver bleeding was allowed for 15 min, and then initial treatment was given by packing the liver injury and infusing fluid for 1 hour before suturing the wound. Then the cold fluid (4℃ lactated Ringer's solution) was infused through the bile duct in the hypothermia group, with no further treatment given to the control group. The body and local liver temperature and hemodynamic variations were recorded. Blood samples were collected from the front vena cava to perform liver function tests. The liver specimens of animals were subjected to histopathological evaluation at the end of the experiment. Results The primary bleeding volume, heart rate and mean artery pressure at different time points were not significantly different between the two groups. After 2 hours intervention, the liver temperature dropped by(1.36±0.80)℃ in the control group and by (2.72±1.01)℃ in the hypothermia group, with significant difference found between the two groups(P=0.020); at the same time point, the body temperature dropped by(0.89±0.76)℃ and (0.97±0.97)℃, respectively; showing no significant difference between the two groups(P=0.87). Liver function examination showed that at the end of infusion aspartate transaminase (AST) in the hypothermia group was significantly lower than that in the control group (P<0.05). The inflammatory cell infiltration of liver tissues in the hypothermia group was slighter than that in the control group. Conclusion Infusion with cold 4℃ lactated Ringer's solution has a protective effect on liver trauma by inducing liver hypothermia to promote the recovery of liver function and alleviate liver inflammation reaction.