Objective To determine whether laser-assisted hatching (LAH) can improve the clinical outcome of frozen-thawed embryo transfers (FET) for patients with previous repeated implantation failure. Methods A total of 97 infertility patients with previous repeated failure, who received assisted reproductive therapy in our in-vitro fertilization(IVF) center from May 2012 to December 2013, were included in this study. A total of 124 FET cycles were randomly divided into LAH group (LAH was performed before embryo transplantation, n=61) and non-assisted hatching group (LAH was not performed before embryo transplantation, n=63). The clinical pregnancy rate,implantation rate, abortion rate and multiple pregnancy rate were compared between the two groups. Results The pregnancy rate (47.5% vs 30.2%,P<0.05) and implantation rate (29.2% vs 19.0%,P<0.05) in the assisted hatching group were both significantly higher than those in the non-assisted hatching group.There were no significant differences between the two groups in abortion rate or multiple pregnancy rate(P>0.05). Conclusion The LAH can increase the pregnancy and implantation rates and improve the outcome of IVF-ET in frozen-thawed embryo transfer cycles for patients with previous repeated failure.