Objective To investigate the effect and safety of flurbiprofen axetil preemptive analgesia for patients undergoing laparoscopic cholecystotomy. Methods Sixty patients of ASAⅠ-Ⅱ undergoing selective laparoscopic cholecystotomy were randomly assigned to two groups: either to receive flurbiprofen axetil 100 mg (flurbiprofen axetil group, n=30)or normal saline 10 mL (control group, n=30) before operation. Postoperative pain was treated by patient-controlled intravenous fentanyl analgesia. The extubation time, recovery time, and sedation-agitation scale (SAS) scores were estimated immediately after anesthesia. Postoperative fentanyl doses, side effects and the visual analgesia scale scores were recorded at 0,2,4,6,12, and 24 h after operation. Results The SAS score, VAS scores immediately after extubation and at different time points after operation in flurbiprofen axetil group were significantly lower than those in control group (P<0.05). The flurbiprofen axetil group had significantly lower total dose of fentanyl (0-24 h after surgery) and significantly less nausea and vomiting than the control group (P<0.05). There were no significant differences in the extubation time and recovery time between the two groups. Conclusion Flurbiprofen axetil preemptive analgesia can alleviate acute postoperative pain of laparoscopic cholecystotomy, with no obvious adverse effect and without increasing the recovery time, making it worth popularizing in clinical practice.