Objective To investigate the role of supervising-nurse in self-protection of medical staffs treating patients with Ebola virus disease in Liberia. Methods Based on the Standard Procedure of Personal Protection, we designed a Chinese-English bilingual Inspection Table for putting on personal protective equipment (PPE) for Chinese People's Liberation Army medical team to Liberia and another one for takingoff PPE. The supervising-nurse gave supervising tips and psychologically positive implications to all medical staffs working in the observing room and treatment ward. Nurses also recorded and summarized the problems and mental status which have been prevented and stopped, and the efficacy of the supervision was evaluated. Results From Jan. 16, 2015 to Mar. 19, 2015, about 3 000 person-times and 126 000 supervision items for the Chinese and Liberia staffs working in the observing room and treatment ward were involved. A total of 1 532 potential problems were prevented by supervising-nurse. Conclusion A supervising-nurse can effectively ensure the self-protection of medical staffs involved in the frontline treatment of patients with Ebola virus disease, guaranteeing the "zero infection" of medical staffs.