Objective To know about the levels of knowledge, attitude and practice of standard isolation precautions among nursing staffs during the anti-Ebola mission in Liberia. Methods The participants were Liberia nursing staffs working in the Ebola Treatment Center in Liberia, which was established by the medical team of the Chinese People's Liberation Army (PLA). An English questionnaire was designed based on the international guidelines on standard isolation precautions, which includes 32 items including general situation, knowledge, attitude and practice on standard isolation precautions. The correlation among the knowledge, attitude and practice of standard isolation precautions was analyzed by Spearman correlation analysis. Results A total of 80 nursing staffs finished the questionnaires, with a valid rate of 71.25%. Their mean scores of knowledge, attitude and practice levels of standard isolation precautions were 7.4±1.1, 36.4±4.6, and 6.6±1.9, respectively. Significant positive correlations were observed between knowledge and attitude (P <0.05), and practice and attitude (P <0.01), but not between knowledge and practice by Spearman correlation analysis. Conclusion The training programs on Ebola virus disease prevention provided by the international health aid organization have improved the isolation precaution behavior levels of the local nursing staffs. However, lack of theoretical training on standard isolation protection and poor supplies from the government lead to enhancement space for attitude improvement on standard isolation precautions in Liberian nursing staff, which may have an impact on the standard isolation precaution behaviors after the international aid staff leaving the country.