Objective To establish a spine solid model of patients using 3D printing technique based on CT tomoscan data, and to apply the model in spine orthopedics. Methods The time for placing the screws, the accuracy rate of screw placement, the incidence rates of nerve and blood vessel injury were retrospectively analyzed and compared before and after 3D printing technique was employed to rebuild the diseased segmental spine in two groups. Results A total of 466 pedical screws were placed in 30 patients who were not subjected to 3D printing technique, and the mean screwing time was (3.1±0.5)min, with an accuracy rate of 85.6% (399/466). While 354 pedical screws were placed in 23 patients who were subjected to 3D printing technique, and their mean screwing time was (2.1±0.4)min, with an accuracy rate of 93.2% (330/354). Significant differences were found for both the screwing time and accuracy between the two groups(P<0.01). No patients had complication such as injuries of the nerve, blood vessel or visceral injury during or after operation. Conclusion Compared to traditional free-hand screwing technique, preoperative 3D printing model of the diseased segmental spine can provide reference for operation planning and screwing, shortening the screwing time and improving the screwing accuracy.