上海市438例体检者同型半胱氨酸水平与代谢关键酶基因多态性的关联分析
CSTR:
作者:
作者单位:

复旦大学 生命科学学院,复旦大学 生命科学学院,仁爱医院检验科,复旦大学 生命科学学院

作者简介:

通讯作者:

中图分类号:

Q31

基金项目:


Correlation of homocysteine levels with gene polymorphisms of metabolic enzyme in 438 adults taking physical examination in Shanghai, China
Author:
Affiliation:

School of Life Sciences Fudan University,,

Fund Project:

  • 摘要
  • |
  • 图/表
  • |
  • 访问统计
  • |
  • 参考文献
  • |
  • 相似文献
  • |
  • 引证文献
  • |
  • 资源附件
  • |
  • 文章评论
    摘要:

    目的 研究上海市438例体检者的同型半胱氨酸(Hcy)水平与代谢相关酶基因多态性的关联。方法 酶循环法检测上海市仁爱医院438例健康体检者的血浆总Hcy(tHcy)、电化学发光法检测叶酸,连接酶检测反应(LDR)进行基因分型。比较不同基因型的Hcy水平,并分析代谢相关酶的基因多态性与tHcy水平的关联。结果 高同型半胱氨酸血症(HHcy)检出率为28.54%(125/438)。MTHFR C677T不同基因型的tHcy水平间差异有统计学意义(P<0.001)。MTHFR A1298C杂合型CA降低HHcy的患病风险[OR=0.49,95% CI:(0.26,0.92),P=0.027]。MTHFR C677T杂合型CT和突变纯合型TT均增加HHcy的患病风险[OR=2.15,95% CI:(1.06,4.36),P=0.035;OR=7.58,95% CI:(3.15,18.22),P<0.001]。MTR G905A、MTRR A66G、MTRR Ac.56+781C、MTR A2756G、CBS C551G并未发现与HHcy的患病风险有关联。结论 MTHFR C677T杂合型CT和突变纯合型TT是HHcy患病的风险基因型;MTHFR A1298C杂合型CA可能有助于降低HHcy风险。

    Abstract:

    Objective To investigate the correlation of homocysteine (Hcy) level with gene polymorphism of Hcy-metabolizing enzymes in healthy adults receiving physical examination in Shanghai, China. Methods Totally 438 participants who were receiving physical examination in Shanghai Ren-ai Hospital were included in this study. The plasma total Hcy (tHcy) levels and serum folate levels were measured by enzymatic cycling methods and electrochemiluminescence, respectively. Genetic typing was determined by ligase detection reaction(LDR). The plasma tHcy levels were compared between different genotypes, and the association of plasma tHcy levels with genetic polymorphisms was analyzed. Results The incidence of hyperhomocysteinemia (HHcy) was 28.54% (125/438) in the present study. There was significant difference in the levels of plasma tHcy among the three genotypes of MTHFR C677T (P<0.001).Heterozygous genotype CA of MTHFR A1298C was associated with a significantly reduced risk of HHcy (OR=0.49, 95% CI: 0.26-0.92,P=0.027); heterozygous genotype CT of MTHFR C677T was associated with a significantly increased risk of HHcy (OR=2.15 , 95% CI: 1.06-4.36, P=0.035), and homozygous genotype TT was also associated with a significantly increased risk of HHcy (OR=7.58, 95% CI: 3.15-18.22, P<0.001). No correlation was found for HHcy risk with MTR G905A, MTRR A66G, MTRR Ac.56+781C, MTR A2756G, or CBS C551G. Conclusion TT and CT genotype of MTHFR C677T have been found to be the risks for HHcy; CA genotype of MTHFR A1298C may help to decrease the risk to HHcy.

    参考文献
    相似文献
    引证文献
相关视频

分享
文章指标
  • 点击次数:
  • 下载次数:
  • HTML阅读次数:
  • 引用次数:
历史
  • 收稿日期:2015-09-22
  • 最后修改日期:2016-04-21
  • 录用日期:2016-08-01
  • 在线发布日期: 2016-08-26
  • 出版日期:
文章二维码
重要通知
友情提醒: 近日发现论文正式见刊或网络首发后,有人冒充我刊编辑部名义给作者发邮件,要求添加微信,此系诈骗行为!可致电编辑部核实:021-81870792。
            《海军军医大学学报》编辑部
关闭